culture media
-Nutrients prepared for microbial growth.
-No living microbes.
-Introduction of microbes into medium.
-Microbes growing in/on culture medium.
agar
-used
as a solidifying agent for culture media in petri plates, slants,& deeps.
difference between
chemically defined media and complex media
In CHEMICALLY DEFINED MEDIA, the exact chemical composition is known.
While in COMPLEX MEDIA, extracts and digests yeasts, meat, or plants.
-Nutrient broth
-Nutrient agar
While in COMPLEX MEDIA, extracts and digests yeasts, meat, or plants.
-Nutrient broth
-Nutrient agar
anaerobic culture media
- REDUCING MEDIA
ANAEROBIC CHAMBER
ANAEROBIC JAR
CAPNOPHILES REQUIRE HIGH CO2
CANDLE JAR
CARBON DIOXIDE PACKET
GENERAL PURPOSE MEDIA
-suppress unwanted microbes and encourage desired microbes.
-make it easy to distinguish colonies of different microbes.
selective and differential media
selective medium
DIFFERENTIAL MEDIUM
- TRIPLE SUGAR IRON AGAR
ENRICHMENT MEDIA
-encourages growth of desired microbe.
- CHOCOLATE AGAR
- BLOOD AGAR
-is a population of cells arising from a single cell or spore
or form a group of attached cells. A.K.A. Colony Forming Unit (CFU).
preserving bacteria cultures
- DEEP-FREEZING (-50C to -95C)
- LYOPHILIZATION (freeze-drying)