-demonstrates enzymes system within the cell Carbohydrates Fermentation Test.
1. TRIPLE SUGAR IRON
indicators:
a. phenol red= acid:Yellow ; alkaline: Red
b. ferrous ammonium sulfate= black (+) for H2S production
A/A: 3 sugars were fermented: Glucose, lactose, sucrose
K/A: only glucose was fermented
K/K: no sugar was fermented
indicators:
a. phenol red= acid:Yellow ; alkaline: Red
b. ferrous ammonium sulfate= black (+) for H2S production
A/A: 3 sugars were fermented: Glucose, lactose, sucrose
K/A: only glucose was fermented
K/K: no sugar was fermented
2. METHYL RED
Must be incubated at 48hours
Positive result: Red
Negative result: Yellow
Must be incubated at 48hours
Positive result: Red
Negative result: Yellow
3. SULFIDE INDOLE MOTILITY
-Sulfide: (+) results= Blackening of the butt
-Indole: (+) results= Red ethereal layer
-Motility: (+) result= growth away from the site of inoculation
-Sulfide: (+) results= Blackening of the butt
-Indole: (+) results= Red ethereal layer
-Motility: (+) result= growth away from the site of inoculation
4. VOGES-PROSKAUER
Must also incubated in 48hours
Positive result: Red
Negative result: Yellow
Must also incubated in 48hours
Positive result: Red
Negative result: Yellow
5. CITRATE UTILIZATION TEST
Medium: Simmon Citrate Medium
pH indicator: Bromcthymol Blue
Positive result: Blue
Negative result: Green
Medium: Simmon Citrate Medium
pH indicator: Bromcthymol Blue
Positive result: Blue
Negative result: Green
6. UREASE TEST
Medium: Christensen Urea Medium
pH indicator: Phenol Red
Medium: Christensen Urea Medium
pH indicator: Phenol Red
7. LYSINE IRON AGAR
Lysine Deaminase: read the slant
(+) Red (-) Violet
Lysine Decarboxylase: read the butt
(+) Violet (-) Yellow
Lysine Deaminase: read the slant
(+) Red (-) Violet
Lysine Decarboxylase: read the butt
(+) Violet (-) Yellow
8. CATALASE TEST
-Uses Hydrogen Peroxide
-Positive for S. aureus
-Uses Hydrogen Peroxide
-Positive for S. aureus
9. OXIDASE TEST
Positive result: change in color
Red colony means the bacteria are viable.
Black colony means the bacteria are dead.
Positive result: change in color
Red colony means the bacteria are viable.
Black colony means the bacteria are dead.